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Western blot analysis of translation of a mutated streptavidin mRNA containing a CGGN1N2 codon (N1 and N2 indicate one of four nucleotides) at the Tyr54 position. (A) In the presence of a nitroPhe-tRNA with a complementary anticodon. (B) In the presence of a non-aminoacylated tRNA with a complementary anticodon.
