Fig. 4.

Treatment with 2076A inhibits HFD-induced obesity. WT (n = 4 for each group) and FXR KO mice (n = 5 for each group) were fed with HFD for 16 weeks and then treated i.p. with 2076A (30 mg/kg per day) for 3 weeks. (A) Representative appearance of mice at the end of the experiment. (B) Representative appearance of visceral of mice. (C) Body weight and body composition analysis. (D) Histologic analysis of the visceral fat by H&E staining. (E–G) The mRNA expression of mitochondrial uncoupling proteins UCPs in the white adipose tissue and skeletal muscle (E), adipocyte differentiation marker genes (F), and lipogenic genes (G) in the visceral fat was measured by real-time PCR. *P < 0.05; ***P < 0.001, compared with the vehicle-treated group. Note only the differences between vehicle group and 2076A-treated group within the same genotype (WT or FXR knockout) were compared, so the unpaired two-tailed Student’s t test was used.