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Sequential usage of the same volume for subsequent ELISA targets. P. falciparum (a) and P. vivax (VK210) (b) ELISA assay results where the same 50 µL homogenate was transferred from the 1st run assay (dark bars) to the 2nd (hatched bars) and subsequently to the 3rd (clear bars) is presented. Indicated in the Y-axes are OD values from absorbance reading at 450 nm and X-axes are individual mosquitoes. Dotted line (b) indicates the OD cut-off value
