Table 1.

Top 5 BLASTP hits for two HO-specific RNA-Seq contigs putatively assigned as TRPA1s exclusive to the HO of adult Dermacentor variabilis from our transcriptome (top) and schematic representation of putative HO-specific TRPA1 partial transcripts in D. variabilis from our transcriptomes aligned with a full-length TRPA1 from C. atrox and a putative TRPA1 from I. scapularis and A. aureolatum using DELTA-BLAST (bottom).¹

Sequence	Desc.	Top 5 Hits	Hit Acc.	E-value	Sim.	Bit-Score	Len.	Pos.
Contig 66838	TRPA1*	A. aureolatum	JAT98721.1	1e-124	98	385	178	174
	TRPA1*	I. scapularis	XP_002434584.1	8e-82	85	252	143	122
	TRPA1*	C. borealis	APG53778.1	1e-55	51	198	167	86
	TRPA1*	H. americanus	APG53784.1	3e-53	50	186	175	87
	TRPA1*	L. anatina	XP_013406001.1	5e-51	48	185	165	79
Contig 70248	TRPA1*	A. aureolatum	JAT98721.1	8e-59	91	204	111	101
	TRPA1*	I. scapularis	XP_002434584.1	2e-43	83	151	81	67
	TRPA1*	C. borealis	APG53778.1	2e-21	44	97.8	112	49
	TRPA1*	S. mimosarum	KFM57166.1	3e-21	43	96.7	116	50
	TRPA1*	P. tepidariorum	XP_015910181.1	7e-21	44	95.9	117	51

¹(*) indicates putatively assigned TRPA1 (i.e. partial transcript, TRPA1 homolog, or TRPA1-like protein); “Desc.” is contig identification assignment; “Hit Acc.” is accession number for match to contig; “Sim.” is similarity score between contig and hit, i.e. the extent to which the two sequences are related; “Len.” is amino acid alignment length between contig and hit; “Pos.” is number of exact amino acid matches between query and subject sequence. The numbers on the schematic (bottom) above the protein sequence illustrations represent amino acid positions. BLASTP = protein-protein BLAST; DELTA-BLAST = Domain Enhanced Lookup Time Accelerated BLAST. Matching organisms: Amblyomma aureolatum, Ixodes scapularis (blacklegged tick), Cancer borealis (Jonah crab), Homarus americanus (American lobster), Lingula anatina (duck mussel), Stegodyphus mimosarum (velvet spider), Parasteatoda tepidariorum (common house spider).