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. 2017 Sep 6;13:72. doi: 10.1186/s13007-017-0222-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Illustration of the specificity of the TaqMan probe designed to target the canonical DQ8-glia-α1 epitope. The high fluorescent signal observed with the α-gliadin clone displaying the canonical epitope (QGSFQPSQQ) combined with the insignificant or absent fluorescence with the clones containing the allelic variants validated the probe specificity. RFU Relative fluorescent units