Fig. 3.

Delayed regeneration after acute injury of muscle in Fmr1 ^−/− mice. a Immunostaining laminin (Lam, green), embryonic myosin heavy chain (embMHC, red) on transverse sections of TA muscle isolated from wild-type (left), and Fmr1 ^−/− (right) mice 10 days after injury. Scale bar, white 50 μm. b Mean myofiber cross-section area (CSA) of wild-type (white) and Fmr1 ^−/− TA muscle 10 days after injury. Numbers of myofibers with greater area than the indicated bin label are shown. c Immunostaining laminin (Lam, red), embryonic myosin heavy chain (embMHC, green) on transverse sections of TA muscle isolated from wild-type (left), and Fmr1 ^−/− (right) mice 21 days after injury. Scale bar, white 50 μm. d Mean myofiber cross-section area (CSA) of wild-type (white) and Fmr1 ^−/− TA muscle 21 days after injury. Numbers of myofibers with greater area than the indicated bin label are shown. e Immunostaining PAX7 (green) laminin (red) on transverse sections of TA muscle isolated from wild-type (upper panel) or Fmr1 ^−/− (lower panel) 21 days after cardiotoxin injury. f Number of PAX7+ satellite cells present underneath the basal lamina, 21 days after injury of the TA muscle of wild-type (white) and Fmr1 ^−/− (gray) mice. Values indicate mean (n ≥ 3) ± s.e.m. *p < 0.05