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. 2017 Sep 6;17:631. doi: 10.1186/s12885-017-3589-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Immunoblotting and immunofluorescence experiments depicting pathways after ABHD5 and DGAT1 siRNA treatment leading to apoptosis and autophagy respectively: a and b- Immunoblotting assay using different antibodies after siRNA transfection with NT (non-target), DGAT1 and ABHD5 siRNA. GAPDH has been used as an internal control. A representative experiment is shown out of the three repeats. c Immunofluorescence assay showing LC3 punctate staining after DGAT siRNA treatment representing autophagy. DAPI (blue)-nucleus and TRITC (red)-LC3. Bar is 50 μm