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. 2017 Sep 6;18:400. doi: 10.1186/s12859-017-1809-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Targeted resequencing assay statistics. Graphical representation of the targeted resequencing assay statistics for five genes (SACS, SETX, APTX, ANO10, CYP27A1) designed using three primer design tools (primerXL, Optimus Primer and Illumina DesignStudio): a Target coverage efficiency, b Distribution of the amplicon sequence and percentage of amplicons harbouring SNPs (c) or secondary structures d in primer annealing sites