Figure 2.
Memory CD4 T cells are also tightly regulated following acutely controlled challenges. (a) Experimental outline to assess the expansion of naive versus memory CD4 T cells following acutely controlled challenges. (b) Summary of the ratio between primary and secondary CD4 T‐cell responses following lymphocytic choriomeningitis virus (LCMV) Armstrong challenge. (c) Number of SMARTA cells in spleen at day 8 after LCMV Armstrong infection. (d) Summary of the ratio between primary and secondary CD4 T‐cell responses following vaccinia‐GP (VV‐GP) challenge. (e) Summary of the ratio between primary and secondary CD4 T‐cell responses following Listeria‐GP61 (LM‐GP61) challenge. 105 naive and 105 memory SMARTA cells from spleen were co‐transferred (1 : 1 ratio) into congenically distinct recipient mice, followed by various acutely controlled antigen challenges 1 day after CD4 T‐cell transfer. SMARTA cells were assessed in peripheral blood mononuclear cells (PBMCs) in all panels unless indicated otherwise. Data are from two experiments, n = 3 or n = 4 mice/group per experiment. Error bars indicate SEM. [Colour figure can be viewed at wileyonlinelibrary.com]