Figure 5.

Endothelial cell adhesion molecules are down‐regulated in the ligated kidney after Fingolimod (FTY720) treatment. Unilateral ureteral obstruction (UUO) mice were treated with saline or FTY720 for 7 days. Proteins were extracted from kidney tissues. Western blot analysis showed that FTY720 suppressed significantly the expression of P‐selectin (a) and vascular endothelial cell VCAM‐1 (a) in the ligated kidneys. The optical densities of P‐selectin (b) and adhesion molecule protein 1 (VCAM‐1) (b) were normalized to that of β‐tubulin. The data are presented as the mean ± standard error of the mean (s.e.m.). *P < 0·05 relative to the control or FTY720‐treated groups. The data represent results from at least three independent experiments. C = contralateral; L = ligated.