Figure 2.

Non‐obese diabetic‐severe combined immunodeficiency‐interleukin (NOD‐SCID‐IL)‐2Rγ^null (NSG) mice injected with human leucocytes develop graft‐versus‐host disease (GVHD). (a–d) NSG mice injected intraperitoneally (i.p.) with either human (h) peripheral blood mononuclear cells (hPBMCs) or saline (control) (day 0) (from Fig. 1) were monitored for clinical signs of graft‐versus‐host disease (GVHD) over 8 weeks. (a) Tissue sections (liver, small intestine and skin) from control (top panel) or hPBMC‐injected mice (bottom panel) at end‐point were stained with haematoxylin and eosin and images captured by microscopy. Each image is representative of two mice per group; bar represents 100 μm. (b–d) The relative expression of murine (m) P2X7 in (b) spleen, (c) liver and (d) small intestine from mice at end‐point was examined by quantitative polymerase chain reaction (qPCR). Data represent group mean ± standard error of the mean (s.e.m.) (n = 3–7); symbols represent individual mice.