Figure 3.

Non‐obese diabetic‐severe combined immunodeficiency‐interleukin (NOD‐SCID‐IL)‐2Rγ^null (NSG) mice express full‐length functional P2X7. (a) Lysates of RAW264.7 (RAW) macrophages and splenocytes from NSG mice were examined by immunoblotting using an anti‐P2X7 antibody. Image is representative of two independent experiments. (b) RAW264.7 cells or splenocytes from C57BL/6, P2X7 knock‐out (KO) or NSG mice in NaCl medium containing 1 μM YO‐PRO‐1²⁺ were incubated in the absence or presence of 1 mM adenosine triphosphate (ATP) for 10 min at 37°C. The mean fluorescence intensity of YO‐PRO‐1²⁺ uptake into RAW264.7 cells or CD11c⁺ splenic dendritic cells (DCs) was then assessed by flow cytometry. ATP‐induced YO‐PRO‐1²⁺ uptake was determined as the difference between YO‐PRO‐1²⁺ uptake in the presence and absence of ATP. Data represent group mean ± standard error of the mean (s.e.m.) (n = 3–5); symbols represent individual replicates (RAW264.7 cells) or mice (splenic CD11c⁺ DCs); ** P < 0·005 and *** P < 0·0001 compared to C57BL/6.