Figure 2.

Enrichment of interleukin (IL)‐17A⁺interferon (IFN)‐γ⁺ and IL‐22⁺IFN‐γ⁺ T cell subsets in the inflamed terminal ileum of Crohn's disease (CD) patients. (a) Total number of isolated living lymphocytes in the CD45⁺lineage⁺ lamina propria mononuclear cell (LPMC) compartment per gram tissue from both unaffected (n = 7) and inflamed groups (n = 13). (b) Representative flow‐plot of the frequency of IFN‐γ⁺IL‐17A⁺, IFN‐γ⁺IL‐17A^–, IFN‐γ⁺IL‐22⁺, IFN‐γ⁺IL‐22^–, IFN‐γ^–IL‐17A⁺ and IFN‐γ^– IL‐22⁺ T cell subsets within CD45⁺lineage⁺LPMC compartment from both unaffected and inflamed terminal ileum. (c) Quantification of the frequency of various T cell subsets within CD45⁺lineage⁺LPMC compartment between unaffected tissue (n = 5) and inflamed tissue (n = 10). (d) Comparison of the fraction of IL‐17A⁺IFN‐γ⁺ T cell subset within IL‐17A‐producers and IL‐22⁺IFN‐γ⁺ T cell subset within IL‐22^–producers between unaffected (n = 5) and inflamed terminal ileum (n = 10). Statistical analyses were performed using a two‐tailed Mann–Whitney U‐test. *P < 0·05; **P < 0·01.