Figure 3.

Germinal centre B (GCB) cell‐intrinsic mammalian target of rapamycin complex 1 (mTORC1) signalling regulated the GCB cell response. (a) Flow cytometry of total lymphocytes in spleens of non‐infected chimeras generated with a mixture of wild‐type (CD45.1⁺) and Aicda‐Cre‐Rptor ^flox (CD45.2⁺) bone marrow cells. The numbers above the outlined areas indicate the percentages of CD45.1⁺ and CD45.2⁺ cells. (b) Flow cytometry of B220⁺ B cells in the LCMV‐infected chimeras in (a), assessed at day 8 after infection of the host with lymphocytic choriomeningitis virus (LCMV), and a summary of GCB cell frequency in B cells of CD45.1⁺ and CD45.2⁺ origin. (c) Flow cytometry of lymphocytes in the LCMV‐infected chimeras in (a), assessed at day 8 after infection of the host with LCMV, and a summary of plasma cell frequency in lymphocytes of CD45.1⁺ and CD45.2⁺ origin. ***P < 0·002 (unpaired two‐tailed t‐test). Data are representative of three independent experiments with three to six mice per group (error bars, SEM). [Colour figure can be viewed at wileyonlinelibrary.com]