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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Cell Rep. 2017 Aug 1;20(5):1088–1099. doi: 10.1016/j.celrep.2017.07.017

Figure 5. The ISS Is Essential for O-GlcNAc Homeostasis in Cultured Colorectal Cancer Cells.

Figure 5

(A) Growth curves of the indicated lines after DMSO (left) or TG treatment (middle, right). Treatment was initiated at day 0. Cells were counted and re-seeded at 0.2 × 105 cells/ml (dashed line) every 3 days.

(B) Representative soft agar assay using wild-type and ISS-deletion clones shown as crystal violet staining or 10× magnified image.

(C) Quantification of soft agar assays. Values are relative to the HCT116 (P) DMSO-treated cells. Data are represented as mean ± SD (n = 3). Statistics compared DMSO to TG treatment.