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. 2001 Sep 1;29(17):3546–3556. doi: 10.1093/nar/29.17.3546

Figure 3.

Figure 3

Figure 3

Myb–DNA complex formation and dissociation: comparative analysis of A-, B- and c-Myb DBDs. (A) Time course of complex dissociation upon competition. Myb–DNA complexes were generated using 40 fmol NR123 from A-Myb (lanes 1–5), B-Myb (lanes 6–10) and c-Myb (lanes 11–15) and 20 fmol MRE-mim1A probe and allowed to form for 15 min at 25°C. The complexes were then exposed to 750 fmol unlabeled MRE-mim1A probe for 0, 10, 20, 40 and 60 min before the samples were analysed by EMSA (as described in Materials and Methods). (B) Time course of complex formation. 40 fmol NR123 of A-Myb (lanes 1–5), B-Myb (lanes 6–10) and c-Myb (lanes 11–15) were incubated with 20 fmol MRE-mim1A for 0.5, 1, 5, 15 and 30 min in the presence of 0.1 µg poly(dI-dC). Then 750 fmol unlabeled MRE-mim1A was added and the samples were incubated for 5 min before loading on a running EMSA gel.