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. 2001 Sep 1;29(17):3546–3556. doi: 10.1093/nar/29.17.3546

Figure 4.

Figure 4

Figure 4

Comparative analysis of DNA binding in vivo by A-, B- and c-Myb DBDs. (A) DNA binding in vivo was estimated using a yeast effector–reporter system (39). An effector plasmid expressing one of the NR123VP-16 fusions A-Myb–VP16, B-Myb–VP16 or c-Myb–VP16 were cotransformed with a lacZ reporter plasmid containing three copies of the MRE-GG element. Transactivation was measured as β-Gal activity after induction by galactose of the effector plasmids expressing the fusion proteins. (B) A western blot shows the expression levels of Myb NR123VP16 fusion proteins as detected by the anti-VP16 antibody 2GV4 in yeast cells transformed with the lacZ reporter and the following expression plasmids as indicated: lane 1, empty vector pDBD11 (–); lane 2, pDBD11-hA-Myb-NR123 (A); lane 3, pDBD11-hB-Myb-NR123 (B); lane 4, pDBD11-hc-Myb-NR123 (C). A non-specific band, serving as loading control, is marked by an arrow.