Table 2.
Recovery and matrix effect for DBS and DBMS assays for 3TC, FTC and TFV.
| Drug | Level | %ME(B/A*100) (SD) | %PE(C/A*100) (SD) | %RE (SD) (C/B*100) |
|---|---|---|---|---|
| DBS | ||||
| TFV | LQC | 107.3 (10.21) | 61.0 (6.03) | 56.9 (2.59) |
| MQC | 105.1 (3.76) | 66.4 (3.02) | 63.1 (2.43) | |
| HQC | 101.9 (2.55) | 69.8 (1.39) | 68.4 (1.71) | |
| Mean (%CV) | 104.8 (2.57) | 65.7 (6.70) | 62.8 (9.20) | |
| 3TC | LQC | 106.33 (4.30) | 73.09 (7.03) | 94.6 (8.11) |
| MQC | 114.52 (5.35) | 90.22 (1.78) | 98.7 (2.78) | |
| HQC | 119.96 (6.96) | 94.84 (3.79) | 83.3 (5.59) | |
| Mean (%CV) | 113.6 (5.54) | 86.05 (4.20) | 92.2 (8.68) | |
| FTC | LQC | 119.4 (8.31) | 56.6 (3.30) | 46.9 (0.64) |
| MQC | 113.4 (4.16) | 63.3 (2.93) | 55.8 (2.49) | |
| HQC | 100.5 (2.82) | 64.8(2.17) | 64.2 (1.41) | |
| Mean (%CV) | 111.3 (8.45) | 61.6 (7.11) | 55.7 (15.0) | |
| DBMS | ||||
| TFV | LQC | 101.3 (4.97) | 59.46 (10.0) | 53.1 (5.89) |
| MQC | 113.1 (5.79) | 44.89 (3.79) | 40.3 (3.30) | |
| HQC | 108.1 (4.97) | 56.52 (4.72) | 52.3 (3.87) | |
| Mean (%CV) | 107.5 (5.51) | 53.6 (14.4) | 48.6 (14.7) | |
| 3TC | LQC | 97.5(7.13) | 57.9 (1.48) | 59.6 (6.21) |
| MQC | 98.5 (8.04) | 60.3 (4.76) | 61.2 (3.64) | |
| HQC | 99.3(6.89) | 75.2 (3.55) | 77.6 (7.63) | |
| Mean (%CV) | 98.5 (0.93) | 64.5 (14.5) | 66.1 (14.9) | |
| FTC | LQC | 88.1 (4.08) | 71.9 (7.58) | 80.2 (6.35) |
| MQC | 84.7 (7.53) | 54.5 (7.40) | 67.2 (8.09) | |
| HQC | 79.1 (4.94) | 68.3 (9.09) | 86.4 (11.5) | |
| Mean (%CV) | 83.9 (5.43) | 64.9 (14.1) | 77.9 (12.6) | |
A = Peak area of aqueous mobile phase solutions without matrix and without extraction; B = Peak area of analyte spiked after extraction; C = Peak area of analyte spiked prior to extraction; %ME = Matrix effect expressed as the ratio of the mean peak area of the analyte spiked after extraction (B) to the mean peak area of an equivalent concentration of analyte in mobile phase (A) × 100; %RE = Extraction yield calculated as the ratio of the mean peak area of the analyte spiked prior to extraction (C) to the mean peak area of the analyte spiked after extraction (B) × 100; %PE = Process efficiency expressed as the ratio of the mean peak area of the analyte spiked prior to extraction (C) to the mean peak area of the same analyte in mobile phase (A) × 100; %CV coefficient of variation (standard deviation/mean × 100).