Figure 4.

P38MAPK Signaling Is Involved in SC-Mediated Tumor Cell Inhibition

UM-SCC-22B (A) or UM-SCC-47 (B) cells were treated with SC (4 μM) or DMSO for 48 hr. Western blot assay was performed to detect the expressions of the indicated proteins or phosphated proteins. Actin was used as the loading control. (C–F) The cells were treated with DMSO, SC (4 μM), SB203850 inhibitor (10 μM), or SC with DMSO or inhibitor (10 μM) together. Then, the relative cell viabilities (C), cell invasion abilities (D), cell migration abilities (E), and miR-21 expression level (F) were determined using the CKK-8 assay, Transwell assay, wound healing assay, and real-time PCR assay, respectively. Data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with control.