Figure 5.

SC Suppressed EMT by Modulating miR-21 Expression

UM-SCC-22B (A) or UM-SCC-47 (B) cells were treated with SC (4 μM) alone or in combination with miR-21 mimics (100 nM) for 48 hr. Western blot assay was performed to determine the protein expression of vimentin or E-cadherin. Actin was used as the loading control. (C and D) Real-time PCR assay was performed to determine the mRNA expression level of vimentin or E-cadherin in both cells. (E and F) Confocal microscopy was used to visualize the vimentin and E-cadherin expression in UM-SCC-22B cells after the same treatment as in (A) and (B). All scale bars, 200 μm. Data are shown as mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 compared with control.