Figure 6.

SC Inhibited UM-SCC-22B Xenografted Tumor Growth in Nude Mice

Tumor-bearing xenografted mice were intravenously treated with SC (5 mg/kg) or saline every other day for 3 weeks. Tumor volumes (A), tumor weights (B), and mice body weights (D) were monitored after treatment. (C) MiR-21 expression level was detected in the tumor tissues at autopsy using real-time PCR assay. (E and F) ALT and AST content from mice blood was analyzed. (G and H) After SC treatment for 48 hr, bioluminescence imaging was performed to monitor the luminescence activity from the tumor-bearing xenografted mice developed by luciferase reporter expressing UM-SCC-22B cells. Representative bioluminescence images and the luciferase reporter scheme are shown. (I) Representative photomicrographs of immunohistochemistry are shown to detect Ki-67, vimentin, or E-cadherin expression on tumor sections after SC treatment. Scale bars, 50 μm. Data are shown as mean ± SD of three independent experiments. **p < 0.01 compared with control.