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. 2017 Jul 1;25(9):2062–2074. doi: 10.1016/j.ymthe.2017.05.022

Figure 3.

Figure 3

Crosstalk between circ-Amotl1 and Stat3

(A) In real-time PCR, downregulation of miR-17-5p and upregulation of fibronectin, Dnmt3a, and Stat3 levels were detected in the circ-Amotl1-transfected cells. **p < 0.01. Error bars denote SD (n = 4). (B) Transfected with circ-Amotl1 increased the expression of Stat3, Dnmt3a, and fibronectin (FN). (C) circ-Amotl1 injection increased the expression of Dnmt3a, fibronectin, and Stat3 in the wound tissues. (D) Top: real-time PCR showed that silencing circ-Amotl1 increased miR-17-5p expression. Bottom: silencing Dnmt3a decreased expression of Dnmt3a and fibronectin. (E) Western blotting confirmed that silencing circ-Amotl1 repressed expression of Stat3, Dnmt3a, and fibronectin. (F) Real-time PCR showed that ectopic expression of Stat3 increased the levels of Stat3, Dnmt3a, and fibronectin but decreased the levels of miR-17-5p. (G) Real-time PCR showed that silencing Stat3 decreased levels of Stat3, Dnmt3a, and fibronectin but increased levels of miR-17-5p. (H) Western blotting confirmed that ectopic expression of Stat3 increased expression of Dnmt3a and fibronectin. (I) Western blotting confirmed that silencing Stat3 decreased expression of Dnmt3a and fibronectin. (J) Western blotting confirmed that silencing Dnmt3a repressed expression of fibronectin but had no effect on Stat3 expression.