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. 2017 Jul 1;25(9):2062–2074. doi: 10.1016/j.ymthe.2017.05.022

Figure 5.

Figure 5

Effects of circ-Amotl1 on Stat3 Nuclear Translocation

(A) A diagram showing that expression of circ-Amotl1 enhanced Stat3 nuclear translocation, which promoted Dnmt3a transcription. Increased Dnmt3a expression repressed miR-17 transcription. (B) Real-time PCR showed that significantly higher levels of circ-Amotl1 were detected in the nuclei compared with the cytoplasm (n = 4). (C) Western blotting showed that ectopic expression of circ-Amotl1 increased Stat3 levels in the nucleus but not in the cytoplasm. (D) Cell nuclear lysis prepared from NIH 3T3 cells was subjected to immunoprecipitation, followed by real-time PCR. Anti-Stat3 antibody pulled down significantly higher levels of circ-Amotl1 when the samples were pre-treated with crosslinking (n = 6). (E) Quantitation of circ-Amotl1, Stat3, Dnmt3a, and fibronectin in wound tissues injected with circ-Amotl1 or the control plasmid. (F) Quantitation of nuclear translocation of circ-Amotl1 and Stat3 in the wound tissues. (G) Quantitation of circ-Amotl1, Stat3, Dnmt3a, and fibronectin in HGF cells transfected with circ-Amotl1 or the vector. (H) Quantitation of nuclear translocation of circ-Amotl1 and Stat3 in HGF cells.