Figure 3.

DNase I footprints of ternary complexes. Nucleosomes containing DS DNA fragments (Nuc) were incubated with increasing amounts of EBNA_452–641 to form ternary complexes, then subjected to DNase I footprint analysis. Bands protected by EBNA_452–641 binding are indicated (arrows and brackets) as are DNase I-hypersensitive bands induced by EBNA_452–641 (*). The DNase I digestion patterns of the naked DS DNA fragment (lane 2) and EBNA_452–641 bound to the naked DS (lane 1) are also shown. The positions of EBNA1 binding sites 1–4 are indicated. (A) and (B) are footprints of opposite DNA strands.