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. 2001 Sep 1;29(17):3520–3528. doi: 10.1093/nar/29.17.3520

Figure 4.

Figure 4

Functional EBNA1 forms a ternary complex with the DS-nucleosome. The DS-nucleosome (Nuc) was incubated with sufficient EBNA1 (7 pmol) to form a ternary complex (lane 4) then this complex was challenged by the addition of a 1000-fold excess of unlabeled DS DNA (DS Comp; lane 5). Complexes were analyzed by EMSA. The positions of the labeled DS DNA (lane 1), the DS-nucleosome (lane 2) and EBNA1 bound to naked DS DNA (lane 6) are also shown. Lanes 1 and 6 contain labeled DS DNA and lanes 2–5 contain the labeled DS-nucleosome.