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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Cell Signal. 2017 May 8;36:154–162. doi: 10.1016/j.cellsig.2017.05.007

Figure 3. ER stress reduces mitochondrial pS727-STAT3 and pFAK.

Figure 3

A) Treatment with TG and TM caused a progressive and pronounced decrease in pS727-STAT3 in the mitochondrial cell fraction, as shown by Western blot (representative of n=3).Treatment for 24 h with the STAT3 inhibitor stattic was used as a positive control. tSTAT3 =total STAT3, which remained stable under ER stress over time. Mitochondrial ATPase was used as a loading control. B) pS727-STAT3 was also reduced in whole cell lysates. A decrease in phosphorylation of FAK on Y397, which is critical for its activation, was seen in largely the same time-dependent manner. tFAK = total FAK, α-tubulin was used as loading control. Blots are representative of n=3. C) Densitometry of blots probed for pFAK/total FAK shows significant decreases in pFAK397 with TG and TM treatment. N=4-5 independent experiments.