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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Cell Signal. 2017 May 8;36:154–162. doi: 10.1016/j.cellsig.2017.05.007

Figure 6. S727-STAT3 protects endothelial cells against ER stress.

Figure 6

STAT3 KO bEnd5 endothelial cells were transfected with GFP-containing constructs and treated with TM for 72 h. Top panels are bright-field images. Bottom images are fluorescent images. A) With control transfections (GFP alone, CON TM), many transfected cells show aberrant small rounded morphology (yellow arrows). B) WT-STAT3-GFP cells looked for the most part healthy, i.e., long-flattened attached cells (white arrows). C) S727A mutants were mostly rounded, whereas D) S727D mutants looked healthy. E) Significantly more GFP+ cells survived TM treatment when overexpressing GFP-WT STAT3 relative to control plasmids. The number of cells transfected with S727A mutants was significantly lower than the WT and S727D mutant transfected cells. Cell counts performed at 72 h are representative of 4 independent experiments, normalized to transfection efficiency obtained at 24 h. * p<0.05 compared to control. # p<0.05, ## p<0.01 compared to S727A.