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. 2017 Jun 23;25(9):2140–2149. doi: 10.1016/j.ymthe.2017.05.018

Figure 4.

Figure 4

CRYAB Is a Target of miR-491 in OS

(A) Sequence alignment of miR-491 with the 3′ UTR of CRYAB genes. (B) miR-491 negatively regulates CRYAB expression and its downstream signaling. MG63 cells that stably express miR-491 or miR-491-antisense were subjected to qRT-PCR and western blot analysis. (C) A 3′ UTR luciferase reporter assay for CRYAB. Wild-type (CRYAB-WT) or mutant 3′ UTR of CRYAB (CRYAB-MT) luciferase reporter constructs were transfected into MG63 cells that stably express miR-491 or miR-491-antisense. After 48 hr of transfection, the luciferase intensity was assessed. (D) The expression of miR-491 and CRYAB was inversely correlated in OS specimens. Using qRT-PCR, we analyzed the expression of miR-491 and CRYAB mRNA in human OS samples (n = 19). *p < 0.05; **p < 0.01; ***p < 0.001.