Fig 6. IHC of primary subcutaneous Kaiso^positive and Kaiso^depleted MDA-231 tumors.

Tumor cells (Tm) of Kaiso^positive (A-C) and Kaiso^depleted (D-F) masses do not invade the epidermis (double-headed arrow in A, D, F, arrow in B). Kaiso^positive tumor cells are labeled strongly positive for Kaiso (A) and vimentin (B) while the Kaiso^depleted cells are labelled considerably less (D, E). The labeling with anti-E-cadherin antibody is negative for both types of tumor cells in contrast to the positive labelling of the mouse epithelium in sebaceous glands (Sb in C, F) and in epidermis (F). Size bars A-F– 50 microns.