Fig 4. Comparison of PrP^C expression among tissue types.

We used one-way ANOVA and Tukey’s Multiple Comparison post-test to analyze differences between PrP^C expression levels in types of tissue (based on ELISA results.) * indicates p<0.05, ** p<0.01, *** p<0.0001. Lower alimentary tissues include: duodenum, jejunum, ileum, cecum, colon and rectum. Ruminant stomachs include: rumen, reticulum, omasum and abomasum. Oropharyngeal lymph nodes include: tonsil, retropharyngeal lymph node, mandibular lymph node and parotid lymph node. Lower GI lymph nodes includes ileocecocolic lymph node and mesenteric lymph node. As indicated by the asterisks, there are statistically significant differences between the following groups: lower alimentary tissues > all lymph nodes; lower alimentary tissues > spleen; ruminant stomachs > spleen; salivary glands > spleen; salivary glands > oropharyngeal lymph nodes. We plotted means and standard error of the mean.