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. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Cell. 2016 Sep 8;166(6):1411–1422.e16. doi: 10.1016/j.cell.2016.08.050

Figure 1. Assembly and characterization of the Med-PIC complex.

Figure 1

(A) Assembly pathway of the Med-PIC complex. Color scheme here and throughout: Mediator Head (aquamarine), Middle (gold), Tail (brown) as well as Pol II (grey), TFIIA (cyan), TFIIB (red), TFIIH (orange), TFIIE (magenta), TFIIF (blue) and TBP (green). (B) Glycerol gradient sedimentation of Med-PIC (upper) and PIC (lower). SDS-PAGE of gradient fractions and of Mediator, TFIIH and TBP-IIB-IIA are shown. (C) SDS-PAGE of the 52-subunit yeast Med-PIC complex (left) and the 21-subunit yeast Mediator complex for reference (right). (D) Transcripts produced from peak gradient fractions for Med-PIC complex and PIC, with and without TBP as indicated, analyzed by gel electrophoresis and autoradiography. Major transcripts are slightly larger than a 73 residue marker (position indicated on the left)