Figure S6.

PBMCs Sample Timeline and T Cell-Neoepitope Recognition Assay, Related to Figures 4 and 5 and Table S5

(A) Blood samples obtained from the patient 550 and 978 days after resection were used for TCR sequencing and T cell – neoepitope recognition assays respectively.

(B) Experimental setup and flow cytometry gating strategy for the T cell –neoepitope recognition assays (intracellular cytokine staining assay) with surface staining of CD3, CD4, CD8, CD45, and intracellular staining of IL-4, IFN-γ, TNF-α. PBMC = peripheral blood mononuclear cells.