Fig 6. The GADD45B SRE function requires HuR, but is only protective against SOX and vhs.

(A, B) 293T cells were treated with siRNAs targeting HuR (A) or NCL (B) or control non targeting siRNAs. 48h later, cells were transfected with the GFP-GADD45B-3’UTR reporter along with a control empty vector or a plasmid expressing SOX. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure GFP mRNA levels. Protein levels of HuR and NCL after siRNA-mediated depletion are shown under the bar graphs (~30% and 45% of total protein left respectively) (C) 293T cells were transfected with the GFP-GADD45B-3’UTR reporter along with a control empty vector or a plasmid expressing the indicated viral endonucleases. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure GFP mRNA levels.