| S. aureus |
|
|
| USA300 LAC |
Wild-type, Erythromycin (ERM) sensitive |
[32] |
| USA300 LACΔselx
|
Deletion of selx, ERM sensitive |
[6] |
| USA300 LACΔselx repaired (rep) |
Reintroduction of selx, ERM sensitive |
[6] |
| USA300 LAC selx EKQD-A |
Re-introduced selx, with site-directed mutations of the sialic acid-binding motif, ERM sensitive |
This Study |
| USA300 spa::Tn |
Transposon insertion into spa
|
[33] |
| E. coli |
|
|
DH5α
|
Cloning strain |
Invitrogen, Paisley, UK |
BL21 DE3
|
Expression strain |
Invitrogen, Paisley, UK |
| Strataclone SoloPack |
lacZΔM15 mutation, endA, recA-deficient |
Agilent Technologies, UK |
