Figure 1.

Mapping the c-Ski interaction domain on Skip. (A and B) Results from representative GST pull-down assays. Purified GST or GST–c-Ski on glutathione resin were incubated with the in vitro translated Skip deletion mutants for 1 h at room temperature. After extensive washing, bound proteins were analysed by SDS–PAGE and autoradiography. (C) A schematic diagram of the Skip deletion mutants used in the analysis. Numbers refer to the amino acid residues and the central red boxed region corresponds to the highly conserved SNW region, where homology with Skip proteins derived from other species is >90%. The relative strength of binding of each protein to c-Ski is also shown, together with the mean percentage binding from at least three separate assays, where +++ and +/– indicate 20–30% and 2–3% binding, respectively.