Figure 3.

Skip is a transcriptional co-activator. U2OS cells were transfected with the HPV-16 E2-responsive pTKM.32 CAT reporter plasmid together with 0.1 or 0.25 µg of pJ4womega.16 E2, as indicated. For assessing Skip co-activation, the input of pJ4womega.16 E2 was fixed at 0.1 µg and increasing amounts of Skip expression plasmid were used as indicated (concentrations are shown in micrograms). After 48 h the cells were harvested and CAT assays performed. (A) Results from a representative assay; (B) collated results from at least three independent transfections. Standard deviations are shown.