Table 1. Summary of K_Ds (in μM units) between CpxI proteins and SNAREΔ60 complexes containing different syntaxin-1 fragments measured by ITC*.

	SNAREΔ60-Sx253	SNAREΔ60-Sx259	SNAREΔ60-Sx265	His6-SNAREΔ60-Sx265
CpxI(48-134)	2.0 [1.4–2.8]	1.4 [1.2–1.7]	2.0 [1.6–2.5]	1.8 [1.5–2.1]
CpxI	1.9 [1.6–2.3]	2.5 [2.0–3.1]	2.4 [2.0–3.0]	2.2 [1.8–2.6]
scCpxI	2.0 [1.8–2.4]	2.2 [1.7–2.5]	2.2 [1.9–2.6]	2.2 [1.9–2.5]

*At least two independent experiments were performed for each combination of CpxI protein and SNAREΔ60 complex. K_Ds were derived from global fit of the independent experiments performed for each combination. For all K_Ds, 68.3% confidence intervals calculated using the error-surface projection method are indicated between brackets.