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. 2017 Aug 10;6:e27190. doi: 10.7554/eLife.27190

Figure 10. Macropinocytosis, trans-endocytosis and cell detachment.

Figure 10.

(A) Macropinosome formation. Sequence shows membrane undulations of an eB1-mCh and mGFP co-expressing cell at the trailing edge. A membrane indentation is pinched off to form an endosome (arrows). Separate panels are shown for mGFP and eB1-mCh, an interpretation of the process at the bottom. (B) Single membrane vesicle. A vesicle in the trailing edge cytoplasm of an eB1-mCh and mGFP co-expressing cell shows eB1-mCh at the outside. An interpretative illustration is shown (right). (C) Double membrane vesicle. A vesicle located in the trailing edge cytoplasm of an eB1-mCh and mGFP co-expressing cell shows label on both sides of the vesicle membrane. An interpretation is shown (right). (D,E) Membrane endocytosis during cell-cell contact resolution. (D) Sequence shows cell detachment of mGFP and ephrinB1-mCh co-expressing cells. A leader cell (upper) resolves its contact (arrows) with a follower (lower) cell. Composite and individual channels are shown. Numerous vesicles are seen at the site of contact during detachment in the leading cell. An interpretation of the process is shown (bottom). (E) TEM of putative trans-endocytotic vesicles. (i) A leading cell presumably undergoing membrane trans-endocytosis as it detaches from a follower cell. A double layered vesicle containing cytoplasmic cargo is indicated (arrow). (ii) Another example of a cytoplasm-filled vesicle within a vesicle (arrow) in the trailing edge cytoplasm. (iii) Various vesicles (arrows) within the trailing edge cytoplasm.