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. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: J Control Release. 2017 Jul 9;261:297–306. doi: 10.1016/j.jconrel.2017.07.014

Fig. 4. The changes of mitochondria membrane potential and membrane integrity of HT-29 human colon cancer cells upon SN-NPM mediated light treatment.

Fig. 4

Cells were incubated with SN-NPM (0.5 μg/mL SN-38 in 10 μg/mL NPM) or PBS for 24 hrs in 96-well black-wall plate, and treated with or without light. Mitochondria membrane potential and cell membrane integrity were stained with 40 nM of DiOC6(3) (Green, ∆Ψm) and propridium iodide (PI, Red) at 24 hrs later post-light treatment, respectively. Nucleus was counterstained with Hoechst 33342. (L, light treatment; PI, propridium iodide; the sacle bar is 100 μm).