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. Author manuscript; available in PMC: 2017 Nov 8.
Published in final edited form as: Oncogene. 2017 May 8;36(36):5189–5198. doi: 10.1038/onc.2017.140

Figure 6. Effect of TSP-4 and SMAD3 shRNA on angiogenesis in response to TGF-β1 in Matrigel plug angiogenesis model.

Figure 6

Matrigel supplemented with TSP-4-, SMAD3-, or control-shRNA-expressing lentiviral particles was injected subcutaneously to WT mice as described in the Methods. TGF-β1 was administered in daily IP injections. A: Quantification of anti-vWF (left panel), anti-α-actin (middle panel), and anti-TSP-4 (right panel) staining in Matrigel plugs of mice injected with Matrigel containing TSP-4 shRNA or control shRNA (n=10). B: Quantification of anti-vWF (left panel), anti-α-actin (middle panel), and anti-TSP-4 (right panel) staining in Matrigel plugs of mice injected with Matrigel containing SMAD3 shRNA or control shRNA (n=10). The stained area was quantified using ImagePro6.1. *p < 0.05 compared to control shRNA, # p<0.05 compared to control (injected with PBS) mice.