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. Author manuscript; available in PMC: 2017 Nov 8.
Published in final edited form as: Oncogene. 2017 May 8;36(36):5189–5198. doi: 10.1038/onc.2017.140

Figure 7. TSP-4 mediates angiogenesis in response to TGF-β1 in a cancer model.

Figure 7

A: Mouse breast cancer EMT6 cells were injected into mammary fat pad of WT or Thbs4−/− (KO) mice. Mice received daily IP injections of TGF-β1. A: representative tumors from one of the experiments. B: tumor weight, n=10, *p < 0.05 compared to control mice injected with PBS; # p<0.05 compared to WT mice. C: Angiogenesis marker CD31 (EC) was visualized by immunohistochemistry in frozen sections of tumors. D: α-actin (SMC) was visualized by immunohistochemistry in frozen sections of tumors from WT and Thbs4−/− mice. E: TSP-4 was visualized by immunohistochemistry in frozen sections of tumors from WT and Thbs4−/− mice. A–D: Mean stained area, % × mean tumor weight, n = 10, *p < 0.05 compared to WT, # p<0.05 compared to WT mice.