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. 2016 Jul 22;8(33):54082–54095. doi: 10.18632/oncotarget.10789

Figure 3. Cytokine-mediated activation of signaling pathways enhances the chemotactic response of primary CD34+ cells to CXCL12.

Figure 3

A. Effect of TPO on CXCL12/CXCR4 signaling in CD34+ cells. TPO (10 ng/mL) was added or not to the upper chamber and the migration in response to CXCL12 (100 ng/mL) was measured in chemotactic assay. B. Starved CD34+ cells were pre-incubated for 2 hours with Ruxolitinib (2 μM) or BEZ235 (10 μM) before stimulation or not with TPO (10 ng/mL). Chemotaxis was then performed in the presence of CXCL12 (100 ng/mL). Data are represented as mean ± SEM.