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. 2016 Jul 22;8(33):54082–54095. doi: 10.18632/oncotarget.10789

Figure 4. Effects of shRNAs against JAK2 WT on the chemotactic response of MO7e cells to CXCL12.

Figure 4

MO7e cells were transduced with two different shRNAs against JAK2 WT (SH3 and SH4) and a control scramble shRNA (SCR). A. Effects of the two shJAK2 (SH3 and SH4) on JAK2 transcripts were evaluated by qRT-PCR. B. Investigation by Western-blot of JAK2 protein expression upon transduction with the two shJAK2. C. Effects of the two shJAK2 (SH3 and SH4) on the phosphorylation of STAT5, AKT and ERK by TPO alone, CXCL12 alone or by combination of both. D. Chemotactic response to CXCL12 (100 ng/mL) of MO7e cells expressing control scramble SCR, SH3 or SH4 shRNA, in the absence or presence of TPO (10 ng/mL). Data are represented as mean ± SEM.