Figure 5. PI3K α and PI3K γ inhibitors abolish the cooperative effect induced by TPO and CXCL12.

MO7e cells were treated for 2 hours with various concentrations (1, 5, 10 or 30 μM) of PI3K inhibitors. A. PI3K α inhibitor (BYL-719), B. PI3K γ inhibitor (AS-252424), C. PI3K β inhibitor (TGX-221), D. PI3K δ inhibitor (CAL-101). TPO (10 ng/mL) was then added or not and chemotaxis was performed in the presence of CXCL12 (100 ng/mL). Data are represented as mean ± SEM.