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. 2016 Jul 22;8(33):54082–54095. doi: 10.18632/oncotarget.10789

Figure 6. Chemotaxis of MF CD34+ cells in response to CXCL12.

Figure 6

A. PB CD34+ cells from 33 MF patients were assayed for chemotaxis in the absence and presence of CXCL12 (100 ng/ml). B. CD34+ cells from mobilized peripheral blood (mPB), non-mobilized PB (nmPB) and bone marrow (BM) samples were assayed for CXCL12-induced chemotaxis compared to MF CD34+ cells. C. CD34+ cells from MF patients were pre-incubated or not with TN140 (5 μM) for 30 min before chemotaxis assays. D. CXCR4 membrane expression and the MFI was determined on gated CD45+/CD34+ cells from mobilized PB (mPB), non-mobilized PB (nmPB), BM and MF PB samples. E. CXCR4 mRNA expression (relative to β2 microglobulin) was quantified by qRT-PCR in CD34+ cells from mobilized and MF patients. F. Correlation between chemotaxis and the overall CXCR4 expression was determined on gated CD45+/CD34+ MF cells. Data are represented as mean ± SEM.