Figure 4. The effect of overexpression of CD164 on the stem cell-like properties.

A. Representative tumor-growth curves of xenografts derived from different cell numbers (n=5, each group). B. Quantification of tumor weight formed by different cell numbers of BEAS2B^CD164 cells. All values were expressed as mean ± SEM. *P < 0.05 indicates statistical significance. C. Representative images of BEAS2B^WT, BEAS2B^CD164 and BEAS2B^sph cells. D. One spheroid cell generated from single-cell culture in 96-well plates. E. Time course of primary sphere formation after culture in ultralow plate for 8 days. The results were the means ± SEMs of three independent experiments. F. Immunoblotting analysis of the indicated proteins in BEAS2B^Veh, BEAS2B^CD164, and BEAS2B^sph cells. G. Flow cytometry for analyzing the expression of CD133 and CD44. H. Immunoblotting analysis of stem cell makers in BEAS2B^Veh, BEAS2B^CD164, and BEAS2B^sph cells. I. The chemoresistance of BEAS2B^sph and BEAS2B^sph cells were performed using MTT assay. All values were expressed as mean ± SEM. *P < 0.05 indicated statistical significance. J. Immunoblotting analysis of ABC transporters in BEAS2B^Veh, BEAS2B^CD164, and BEAS2B^sph cells.