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. 2017 Apr 21;8(33):54285–54296. doi: 10.18632/oncotarget.17348

Figure 5. Inhibition of APOBEC3G increased the radiosensitivity of GBM cells and mesenchymal GICs.

Figure 5

(A–B) Colony formation assay showed that APOBEC3G knockdown reduced the colony formation of A172 cells after IR (2 Gy). A172-SCR and A173-shAPOBEC3G were treated with or without IR (2 Gy) before being allowed to recover and grow for 10 days. Representative pictures are illustrated (A) and colony number was counted from triplicate experiments (B). (C) Limiting dilution sphere formation assay demonstrated that targeting APOBEC3G reduced sphere formation in GSC20 cells after IR (2 Gy). GSC20 cells were targeted with scramble or APOBEC3G shRNA, treated without or with IR (2 Gy), and diluted; 1-3 cells were plated and allowed to recover and grow for 21 days. Sphere-positive wells were counted, and the percentage of positive wells was calculated. (D–E) Immunoblot analysis showed that APOBEC3G knockdown impaired the DNA repair response after IR. A172 cells (D) and GSC20 (E) with scramble or APOBEC3G shRNA were exposed to 2 Gy IR. Cells were harvested before IR or 3 h or 12 h after IR for an immunoblot analysis with specific antibodies against phosphorylated Chk2, total Chk2, Cleaved-PARP, and APOBEC3G.