Skip to main content
. 2017 Apr 13;8(33):54478–54496. doi: 10.18632/oncotarget.17091

Figure 5. Short exposure to acidosis induces the release of inflammatory and nociceptive mediators in mesenchymal stromal cells of the BM microenvironment (MSCs, HOBs, and CAFs).

Figure 5

(A) mRNA analysis of inflammatory and nociceptive mediators. Mean ± SE (N = 3 lots with N = 2 technical and N = 2 biological replicates each lot for MSCs and HOBs, and only 1 lot for CAFs with the same number of biological and technical replicates) ***p<0.001, **p < 0.01, and *p < 0.05; (B) mRNA expression of BDNF and NGF in bmMDA cells in respect to HOBs exposed for 24 hrs to low pH (= K, 100%); (C) ELISA quantification of IL6 and IL8 in the culture supernatants. Mean ± SE (N = 3 lots with N = 2 technical each lot for MSCs and HOBs, and only 1 lot for CAFs with N = 2 technical and N = 2 biological replicates) **p < 0.01 and *p < 0.05.