Table 3. Used antibodies and positivity thresholds for immunohistochemistry.

Antigen	Antibody	Dilution	Stratification of staining results
PSCA	MB1[4]	5 ng/μl	1-9% weak positiv; >9% strong positiv
Ki-67	MIB-1 (DAKO)	1:50	0-9%, 10-20%, >20%
estrogen receptor	SP1 (Ventana)	prediluted by manufacturer	threshold for positivity: >1%
progesterone receptor	1E2 (Ventana)	prediluted by manufacturer	threshold for positivity: >1%
HER-2/neu	A0485 (DAKO)	1:800	negative: IRS 0, 1+, 2+ without Her-2/neugene amplificationpositive: IRS 2+ with Her-2/neu geneamplification, IRS 3+; [51]