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. 2017 May 2;8(33):54604–54615. doi: 10.18632/oncotarget.17554

Figure 1. Assesment of functional basal autophagy in vitro.

Figure 1

(A) Immunoblotting of LC3B and p62 upon pharmacological autophagy inhibition. A dose-dependent increase of LC3B and p62 was observed with increasing concentrations of chloroquine. (B) Immunofluorescence of LC3B and p62 upon pharmacological autophagy inhibition. I. In CC cell lines HT-29 and II. LoVo a dose-dependent increase of LC3B and p62 was observed with increasing concentrations of chloroquine. (C) Electron microscopy of CC cell lines pharmacological autophagy inhibition. I-III. In CC cell line HT-29 a dose-dependent increase of cytoplasmic vesicles was observed with increasing concentrations of CQ.